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How to read a Certificate of Analysis — beginner guide

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Posts: 33
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(@brandwatcher)
Eminent Member
Joined: 5 months ago
[#147]

People keep asking how to evaluate a CoA. Let me break down what to look for in a mad honey CoA specifically:

Header section:
- Lab name and accreditation (ISO 17025 is the relevant standard)
- Report date
- Sample ID (this is what you match to your order's batch number)
- Product description (should reference honey and grayanotoxin/diterpenoid testing)

Results section:
- Grayanotoxin value in mg/kg — what you're actually there for. Look for a specific number, not just "detected" or "compliant"
- Which GTX variants were tested (GTX-I and GTX-III are most relevant)
- Detection limit listed — if the value is near the detection limit, precision is lower
- Method: HPLC is standard; HPLC-MS is better

Red flags:
- No specific grayanotoxin number, just "compliant" or "tested"
- Lab name you can't find with a basic search
- Date that predates the claimed harvest season
- No sample ID, or sample ID that doesn't match your order


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Posts: 12
(@eurolex)
Active Member
Joined: 5 months ago

Good guide. One addition: the difference between HPLC and HPLC-MS (mass spectrometry) matters. Plain HPLC can measure total content reasonably well but HPLC-MS gives you better specificity for individual GTX variants and is significantly harder to fake convincingly. If you see an HPLC-MS CoA from an ISO 17025 accredited lab with a named analyst, that's as good as it gets in terms of documentation quality.


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